Effects of Humate and Organic Based Soil Treatments on Urban Soil Characteristics
Zack Shier, Board Certified Master Arborist and Plant Health Care Manager at Joseph Tree Service, is utilizing microBIOMETER® in his study titled Effects of Humate and Organic Based Soil Treatments on Urban Soil Characteristics.
Introduction to the study. Urban soils have long plagued tree care providers with a difficult obstacle to tree health optimization. The very nature of how our urban soils come to be makes it quite challenging to diagnose the major issues with our soils, let alone correct those issues consistently, and with enough efficiency to make it affordable to clients.
When buildings or homes are built in our cities and towns, the natural layout and structure of soils is heavily modified, and often changed in erratic ways. Large holes are dug, bringing soil horizons meant for the deep areas, to the surface; mixing heavily with surface horizons. The top O and A soil horizons are often scraped clean to level surfaces, moving them or completely taking them away. Outside products, like “clean-fill” are often brought in, adding foreign soil or even rock (like quarry, limestone fill) into the surface soil area.
On top of this sub-par growing medium we’ve created, we also plant turf or put asphalt and concrete into most of the area. We then rake up and get rid of all organic litter and material, continually robbing the soil of the reincorporation of organic matter that forests are accustomed to. To add to the problem, urban trees are grown quickly using synthetic fertilizers on tree farms, and then dug up to be planted, cutting anywhere between 50-90% of their roots off, and often planted in different soil than they were grown in.
This entire predicament creates poor chemical, physical, and biological soil characteristics, resulting in poor urban tree growth, increased insect and disease populations, and high rates of nutrient deficiencies. (Read more)
microBIOMETER® can tell you if you are increasing the nutrient value and disease resistance of your crop.
A Rodale study showed greatly increased levels of the vitamins and minerals in sustainably farmed soils as opposed to mineral fertilized crops. And at Rodale, the sustainable practice yields are the same as the paired fields farmed with mineral fertilizers – and in bad weather, and disease years significantly better. Rodale is only one of many studies showing the increased nutrient value of organically and sustainably grown food.
Now Dr. Montgomery of the University of Washington’s team in a similar study has shown that if you are increasing your microbial biomass you are increasing the nutrient level of your crop: “soil health is a more pertinent metric for assessing the impact of farming practices on the nutrient composition of crops”.
Biklé, A. and Montgomery, D.R., 2021. Soil health and nutrient density: beyond organic vs. conventional farming. Frontiers in Sustainable Food Systems.
Hepperly, P.R., Omondi, E. and Seidel, R., 2018. Soil regeneration increases crop nutrients, antioxidants and adaptive responses. MOJ Food Process Technol, 6(2), pp.196-203.
Nature article reports that microbial biomass estimates by microBIOMETER® correlates with soil health and yield stability.
The microBIOMETER® soil test was used to report microbial biomass in a recent Nature publication*. Scientists Dr. Judith Fitzpatrick and Dr. Brady Trexler of microBIOMETER® collaborated with a University of Tennessee team headed by Dr. Amin Nouri. The team evaluated the effects on soil health and yield stability of 39 different methods of raising cotton over 29 years. The conditions tested included till, no-till, various cover crops and different levels of nitrogen fertilization.
The study found that the major impacts on yield were very dry or wet conditions, and low or high temperatures. The deleterious effects of these weather extremes on yield were mitigated by regenerative agricultural practices which resulted in adequate soil, C, N, soil structure and microbial biomass.
*Nouri, A., Yoder, D.C., Raji, M., Ceylan, S., Jagadamma, S., Lee, J., Walker, F.R., Yin, X., Fitzpatrick, J., Trexler, B. and Arelli, P., 2021. Conservation agriculture increases the soil resilience and cotton yield stability in climate extremes of the southeast US. Communications Earth & Environment, 2(1), pp.1-12.
PLANT Group is a team of designers, engineers, and ecologists. The company is building systems to connect humans and nature. In the process, they are soil testing at a hemp farm in Iowa, Honeysuckle Hops & Hemp.
First, through their partnership with Blue Forest Farms, the team at PLANT Group is using microBIOMETER®. They are utilizing the tool to research soil health and carbon sequestration implications of growing hemp.
Furthermore, Austin has an interest in publishing research on their alternate organic hemp production methods. microBIOMETER® is assisting them in collecting some “pretty cool data on microbial biomass and fungal ratios in the soil in response to these strategies.”
Meanwhile, the company recently launched a new line of hemp food products, Hemp Hearts. They grow each plant regeneratively. This includes a focus on soil carbon, biodiversity, and ecosystem health.
Did you know one hectare of hemp can absorb 15 tons of CO2 per hectare? Hemp’s rapid growth makes it one of the fastest CO2-to-biomass conversion tools available. Therefore, let’s stop cutting down our forests. Plant some hemp instead!
Lastly, please visit the PLANT Group website to meet the rest of the team and learn more about their business. And follow them on Instagram to keep up to date on their progress!

“Last weekend I had the privilege to teach community gardeners on the importance of soil testing side by side with my very first student at NYBG Adult Education program (class 2015). Dr. Joan Basile is a clinical psychologist who has developed her own horticulture therapy program incorporating soil knowledge brining therapy & soil science & gardening together.” – Dr. Anna Paltseva, soil_expert.
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“While the microBIOMETER® results showed there is room for improvement, the result from last year’s beds also proved that composting and mulching practices are paying off in increased soil life. This means that sandy soil will gradually be able to hold nutrients better and better!” – Dr. Basile

“One of our primary objectives is that farmers succeed in putting biology back into their soils to ensure their natural fertility. We are therefore very interested in everything that lives in the soil, from earthworms and microarthropods to microorganisms (bacteria, fungi, nematodes). For us, microbial biomass is one of the most important indicators that help us understand soil biology. Fungal to bacterial ratio, which is a less documented indicator for the moment, remains interesting to observe in certain situations and is the object of real research by our R&D team to understand how best to interpret it.
We have been using microBIOMETER® for 8 months now to test the soil in different projects in our panel of biological indicators. microBIOMETER® provides us with quick and easy results on microbial biomass and F:B ratio which is a real plus for us. We can perform tests directly in the field and present the results to the farmers. Moreover, the affordable price of the analysis allows us to perform soil biology tests in projects where we had no affordable way to do so before.”
*CDA, Centre de Développement de l’Agroécologie, are affiliates dedicated to R&D and advisory.
Ariel White, a ninth grader at Pretty River Academy in Ontario, Canada, utilized microBIOMETER® in their science fair project titled Post Wildfire Forest Reboot Kit.
The project was awarded first place at their high school and chosen to compete at the Simcoe County Regional Science Fair. At the regional fair, Ariel was awarded a gold medal, Best of Earth and Environmental Sciences, Best of Fair, The Dufferin Simcoe Land Stewardship Network Award, and was one of seven students selected to represent their county in the Canada Wide Science Fair where they won a silver medal!
About the project: Forest fires have increased due to climate change, causing forests to burn down at an unbelievable rate. Now we need forests more than ever, yet they have been taking years if at all to regrow. This project explores the question “how can we boost the speed of forest regrowth after forest fires?”. For phase one of this experiment, each plant was graded for performance using tests such as success-rate, growth-rate, compost-value, and self-propagation. For the second phase, it was seen what effect this plant had on the soil microbiome; which is key to healthy, speedy plant growth and isn’t evident after fires. It was concluded that the morning glory substantially increased the microbiome health from inevident to healthy, and had an almost perfect performance score. These results are very important to our world’s future as they could help to deter climate change and repair our forests and their diverse ecosystems.
Ralph Lett, head of product development at Acterra, was kind enough to share his microBIOMETER® experience with us. We love hearing the different ways our customers are using our soil test! Please contact us if you would like to share your soil testing story.
“Thanks for taking an interest in how our company is using microBIOMETER®.
Acterra is a bio stimulant company. We work closely with our sales partner, Bio-Active. Together we capture and solubilize diesel emissions in a fusion tank and then add a beneficial consortium of facultative microbes . This is revolutionary as it allows the farmer to make his own biological fertilizers while he is seeding and/or harvesting.
microBIOMETER® is a handy tool for us to measure the microbial weight of our bio fertilizers when we come in off the fields to refill our tanks . The microbes in our fusion tank reproduce incredibly quickly, much faster than a regular brewing process. If the microbial populations grow too quickly things can get plugged up and can cause problems. This is where microBIOMETER® is incredibly useful. We use it to keep an eye on the populations in our tanks so that if over populations occur we can quickly drain the tank and start over .
We plan to continue working with microBIOMETER® in the future. Our hope is that one day farmers will be able to sit in their tractors and know exactly the microbial bio weight of their biological fertilizers while they are farming their fields.
Never stop innovating !” – Ralph Lett
Last semester Soil Science and Environmental Pedology students under a supervision of Dr. Anna Paltseva [annapaltseva.com] conducted a soil microbial experiment on campus of University of Louisiana at Lafayette. First, each of the group of students collected different samples. Samples were collected from lawn, vegetable containers, around tree pits, and a native plants garden. The soil samples were analyzed in accordance with the provided procedure, which is simple and fast. The microBIOMETER® app tested the samples and gave each of the readings. The readings were in microbial biomass expressed in microbial-carbon per gram of soil (ug/g) and fungal to bacteria ratio F:B, F% and B%.
The vegetable container and samples from the tree pits showed the highest range of 400 – 800 ug/g. This is due to high organic matter content. The low results from the lawns may have been due to limited microbiological diversity due to monoculture of grasses grown. The areas close to the water bodies (culvert, pond, etc.) may have been lower due to organic matter washing away over time. All the samples were predominantly rich in bacterial population over fungal.
“microBIOMETER® is a very efficient and time saving tool. It can be used by scientists, farmers, or a gardener in learning the microbial health of their soil. This testing process is also very non-invasive, and thus having a lesser negative environmental impact compared to traditional testing. One of the students said, It was pretty cool using an app to analyze soil. I want to know how it works since it all felt like magic.” – Dr. Anna Paltseva
Click here to view the student’s video.
Order a microBIOMETER® Academia Kit for soil testing in your classroom!
If you are interested in Dr. Paltseva’s research or would like to learn more about urban soils, please follow her on Instagram.

Microbial Biomass Chart
Ben Taylor-Davies, also known as Regen Ben, is a farmer and bioagri-ecologist working from Herefordshire in the UK. His farm is based in Ross-on-Wye and has been focused on environmental improvements for the past 22 years. His work includes creating 12km of new hedges with 6m of pollen and nectar or ground bird nesting margins around every field as well as working on river meadow restoration.
Following a Nuffield scholarship in 2016 and the opportunity to travel the world (USA, Canada, Brazil, Argentina, Uruguay, Paraguay, Chile, Peru, South Africa, France, Belgium, Germany, Poland, Ukraine, Belarus, Russia, Mongolia, China, Singapore and Australia), Ben was intrigued by the regenerative agriculture movement which very much complimented the environmental work he was doing back on his own farm. When discussing these soil health focused farming methods with clients as an agronomist, it struck a chord with many of them too; the future of agriculture and real farm sustainability.
Ben came across microBIOMETER® in 2019 and found it an incredibly useful tool in benchmarking clients farms in order to start monitoring change in what they were doing. The real time results offered by microBIOMETER® provides Ben with full control over how, where and when he takes readings. Ben uses his microBIOMETER® readings in conjunction with the What3words app which allows him to accurately repeat measurements in subsequent years in order to build a picture of successes and failures.